Oligonucleotide synthesis

 

 

Contact us in any
of the following ways:

 

tel: +48 (012) 266 39 10

fax: +48 (012) 266 39 11

mobile: +48 0 507 470 880

biote21@biote21.com

 

We would love to hear
from you!

 

Synthesis of long double strand DNA (FRAGMENTS)

 

Chemical synthesis of ssDNA oligonucleotides – standard oligonucleotides for PCR reaction.

Result: The product of synthesis (6-80 alkalis) will be issued in a form of purified oligonucleotide (in situ collection or mail delivery).

 

 

Synthesis of standard oligonucleotides  -price list ( PLN/ nucleotide, net)

Purification

Synthesis scale
0,04 [uM]

Synthesis scale
0,2 [uM]

Synthesis scale
1 [uM]

Desalting (sephadex)
- standard*

1,60

2,60

6,40

DMT-on columns*

3,80

4,80

9,20

Purification HPLC*

4,60

5,60

10,60

*BioTe21 guarantees efficiency +/- 30% in relation to standard 20- nucleotide synthesis product after standard purification (desalting). Application of additional non-standard purification may result in rapid drop in guaranteed cost-effectiveness.

 

For orders over 80 alkalis the price increases by 50%.
Details on discounts further down in a link: Special offer.

 

Chemical synthesis of RNA oligonucleotides:

 

  • siRNA
  • miRNA, anty-miRNA
  • antisenses
  • dsRNA
  • 2’-0-methyl ssRNA( resistant to RNAz)

 

Synthesis of RNA oligonucleotides  -  price list [ PLN/ nucleotide, net]

Purification

Synthesis scale
0,2 [uM]

Synthesis scale
1 [uM]

Desalting (sephadex)

- standard*

20,60

32,40

Purification HPLC*

45,20

65,60

* Results: the product of synthesis (up to 60 alkalis) will be issued in a form of purified oligonucleotide (collection in situ or mail delivery).

 

BioTe21 guarantees efficiency +/- 30% in relation to standard 20- nucleotide synthesis product after standard purification (desalting). Application of additional non-standard purification may result in rapid drop in guaranteed cost-effectiveness

 

Modified oligonucleotides: (o to Pan nie prosił, ale znalazłam do dorzucam)

 

The synthesis of labelled oligonucleotides DNA and RNA (a synthesis of molecular probes):

  • Single labelling with fluorochromes 3’ and 5’
  • Dual labelling: a fluorochrome – non-fluorescent quencher
  • Internal labelling with fluorochromes
  • Binding of synthetic ssDNA and ssRNA with proteins

 

The result:

The synthesis product will be issued in a form of purified oligonucleotide (personal collection or mail delivery). The price of modified oligonucleotide includes price of non-modified oligonucleotide with regards to various purification options (compare the offer of standard oligonucleotides synthesis) along with price of introducing a modifying group/reagent into a oligonucleotide (below). In case of synthesis of molecular probes such as molecular beacons, dual labeled fluorogenic probes and other probes of FRET type(i.e. for Real Time PCR reaction) HPLC as a method of purification is recommended./has to be chosen.

 

Price offer of the synthesis with introduction of standard modifying group

scale of synthesis 0,2 [uM]*

Modifying group/ reagents

Labelling 5’

Labelling 3’

Internal labelling

5-FAM / FAM

165

 

(dT) 450

TET

190

 

 

HEX

190

 

 

JOE

370

 

 

ROX

380

 

 

Cy3/Cy5

320

 

 

Pulsar R 650

 

390

 

TAMRA

240

290

 

BHQ-Q

 

390

 

BHQ-1

 

350

 

BHQ-2

 

390

 

BHQ-3

 

390

 

Aminolink 18 (spacer)

220

 

220

Biotin

150

 

 

 

*Price offer for 0,04[uM] scale is 25% cheaper than for standard 0,2 [uM] scale whereas prices for scale 1 [uM] are 40% higher in comparison to standard scale.

We guarantee +/- 30% efficiency with respect to standard 20-nucleotide synthesis product after standard purification (desalting). Application of additional non-standard purification may result in bringing down guaranteed efficiency.

Delivery time for oligonucleotides labelled with standard modifying groups/reagents - within 8 working days. There is a possibility to request rare modifying groups/reagents (not listed in the table) and introduce them into a nucleotide. The term of realisation will be prolonged in accordance with delivery time of requested reagents. In each case, please contact our laboratory. With orders of oligonucleotides above 80 alkalis the price of synthesis increases by 50%. Details on our discount –further down in a link: Special offer.

 

Starters and fluorescently labelled molecular probes design

 

Our company offers projects of molecular probes (i.e. molecular beacons, scorpions etc.) on the basis of provided array sequences, for which the probes are to be designed, and information related to detection method (i.e. type of apparatus for Real Time PCR reaction). A project includes 1-3 options of thermodynamically analysed probes’ sequences along with a set of fluorochromes/ quenchers suitable for a chosen research method. In the case of each order please contact our staff.

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